The Biological bulletin (19752403094)

Title: The Biological bulletin

Identifier: biologicalbullet108mari (find matches)

Year: [1] (s)

Authors: Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983

Subjects: Biology; Zoology; Biology; Marine Biology

Publisher: Woods Hole, Mass. : Marine Biological Laboratory

Contributing Library: MBLWHOI Library

Digitizing Sponsor: MBLWHOI Library

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48 W. H. McSHAN, SOL KRAMER AND N. F. OLSON and Potter, 1943). The p,g. of phosphorus released in 15 minutes by the different amounts of tissue were used for calculating the //.g. of phosphorus hydrolyzed by 1 mg. of tissue. The values used for preparing the graphs and given in the tables are expressed in terms of the /u.g. of phosphorus released by 1 mg. of muscle in 15 minutes. These values represent the number of ATPase units per mg. of muscle. RESULTS Relation of ATP concentration to enzyme activity Maximum ATPase activity was obtained when 2 mg. of muscle tissue were incubated with as low as 1.4 X 10~3 M ATP. In order to insure an excess of substrate a final concentration of 2.8 X 10~3 M ATP was used in the system for the routine determinations. oWITH Co*"1" • WITHOUT Co** o WITH Ccf* • WITHOUT Co+t FEMALE AMERICAN COCKROACH MALE AMERICAN COCKROACH

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1.0 MG TISSUE 1.0 MG TISSUE FIGURE 2. Effect of Ca ions on the release of inorganic phosphorus from ATP by the ATPase of muscle from the male and female cockroach. Effect of time and enzyme concentration The rate at which inorganic phosphorus was released from ATP by different amounts of tissue was not constant with time. This decrease in ATPase activity with time has been reported for liver homogenates (Novikoff et al., 1952), for purified yeast ATPase (Meyerhof and Ohlmeyer, 1952), and for mitochondria of flies (Sacktor, 1953). The results given graphically in Figure 1 for the muscle of the female cockroach and female woodroach show that the amounts of inorganic phosphorus released were linearly related to the enzyme concentration when 0.5, 1.0, 1.5 and 2.0 mg. of

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