Madonna and Child Enthroned with Saints Peter, Bartholomew, Catherine of Alexandria, and Paul, and (below) the Nativity; left wing (top to bottom): Annunciatory Angel, Crucified Christ with the Virgin, Saints Mary Magdalen and John, and Christ as the Man of Sorrows; right wing (top to bottom): Virgin Annunciate, Saints Onophrius and Paphnutius, and Saint Onophrius Buried by Saint Paphnutius.

Italian Renaissance painting is most often be divided into four periods: the Proto-Renaissance (1300–1425), the Early Renaissance (1425–1495), the High Renaissance (1495–1520), and Mannerism (1520–1600). The city of Florence is renowned as the birthplace of the Renaissance, and in particular of Renaissance painting. From the early 15th to late 16th centuries, Italy was divided into many political states. The painters of Renaissance Italy wandered Italy, disseminating artistic and philosophical ideas. The Proto-Renaissance begins with the professional life of the painter Giotto and includes Taddeo Gaddi, Orcagna and Altichiero. The Early Renaissance style was started by Masaccio and then further developed by Fra Angelico, Paolo Uccello, Piero della Francesca, Sandro Botticelli, Verrocchio, Domenico Ghirlandaio and Giovanni Bellini. The High Renaissance period was that of Leonardo da Vinci, Michelangelo, Raphael, Andrea del Sarto, Coreggio, Giorgione, the latter works of Giovanni Bellini, and Titian. The Mannerist period, dealt with in a separate article, included the latter works of Michelangelo, as well as Pontormo, Parmigianino, Bronzino and Tintoretto.